Journal: Journal of Nanobiotechnology

Article Title: Injectable platelet-mimicking silk protein-peptide conjugate microspheres for hemostasis modulation and targeted treatment of internal bleeding

doi: 10.1186/s12951-025-03180-w

Figure Lengend Snippet: Metabolic distribution and biocompatibility of hemostatic microspheres in vivo. ( A ) Bioluminescence imaging illustrates the metabolic distribution of free Cy5 and Cy5-labeled microspheres in various metabolic organs 36 h post-injection. Cy5: free Cy5; SFMP: Cy5-SFMP; RDMP: Cy5-RDMP; LDMP: Cy5-LDMP; TIMP: Cy5-TIMP. ( B ) Immunofluorescence co-staining of tissue at the injection site, demonstrating the presence of inflammatory cells, highlighted by CD68 (green), and the pro-inflammatory cytokine TNF-α (red), with DAPI (blue) marking the cell nuclei. ( C ) H&E staining was performed on the collected tissues 14 d after injection of the hemostatic microspheres

Article Snippet: The tissue samples were first incubated with CD68 primary antibody (1:200, Booster, BA3638) and TNF-α primary antibody (1:800, Bioss, bs-10802R), followed by incubation with TYR-488 (PN0100, Pinuofei Biological) and TYR-555 (PN0101, Pinuofei Biological), respectively.

Techniques: In Vivo, Imaging, Labeling, Injection, Immunofluorescence, Staining

Journal: Experimental eye research

Article Title: A modified high-yield method for primary culture of rat retinal microglial cells.

doi: 10.1016/j.exer.2021.108919

Figure Lengend Snippet: Fig. 4. Primary rat retinal microglial cells were activated by LPS stimulation. (A) Microglial cells were treated with LPS (100 ng/mL) for 8 h on day 2. Phase-contrast microscopy images showed an abrupt change in morphology from ramified state (red arrow) to amoeboid (red arrowhead) after LPS stimulation; Scale bar: 100 μm; (B) Representative immunofluorescent images of primary retinal microglia stained with CD68 (red), and DAPI (blue). The expression of CD68 was upregulated after LPS (100 ng/mL) treatment for 8h; Scale bar: 50 μm. (C) Representative immunofluorescent images of primary retinal microglia with fluorescent beads (green) and nuclei labeled with DAPI (blue); microglial cells with phagocytosed beads were increased after LPS stimulation for 4h. The experiment was performed three times. Scale bar: 50 μm. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The primary antibodies used in this study were rabbit anti-IBA1 antibody (Abcam, ab178847, 1:500), rabbit anti-CD68 antibody (Boster, BA3638, 1:200), rat anti-CD11b antibody (Abcam, ab8878, 1:500), rabbit anti-GFAP antibody (Abcam, ab7260, 1:200), and rabbit antiTMEM119 antibody (Proteintech, 27585-1-AP, 1:200); the nuclei were stained with DAPI (Beyotime Biotechnology, C1002).

Techniques: Microscopy, Staining, Expressing, Labeling

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Immunohistochemistry staining for PD-L1, CD68, and CD163 expression in cervical squamous cell carcinoma tissues. The left panel shows negative staining for PD-L1 and low macrophage density. The right panel shows positive staining for PD-L1 and high macrophage density.

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Immunohistochemistry, Staining, Expressing, Negative Staining

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Density of CD68- and CD163-Positive Cells in the Tumor Field and Clinical Features in 120 Samples of Cervical Squamous Cell Carcinoma

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Biomarker Discovery

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Correlation of PD-L1 expression and density of CD68-and CD163-positive cells in cervical cancer. Notes: ( A ) PD-L1 expression in tumor cells and density ofCD68. ( B ) PD-L1 expression in tumor cells and density of CD163. ( C ) PD-L1 expression in the stroma and density of CD68. ( D ) PD-L1 expression in the stroma and density of CD163.

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Relationship between the density of CD68- and CD163-positive cells and the expression of PD-L1 in cervical cancer. ( A ) Density of CD68 in PD-L1 positive and negative expression groups in TC. ( B ) Density of CD163 in PD-L1 positive and negative expression groups in TC. ( C ) Density of CD68 in PD-L1 positive and negative expression groups in stroma. ( D ) Density of CD163 in PD-L1 positive and negative expression groups in stroma.

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Univariate and Multivariate Analysis of Factors Associated with PD-L1 Expression in Tumor Cells

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing, Biomarker Discovery

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Recurrence-free and overall survival curves in CC patients. ( A ) Kaplan–Meier OS curves according to PD-L1 expression in tumor cells ( B ) Kaplan–Meier OS curves according to PD-L1 expression in stroma ( C ) Kaplan–Meier OS curves according to the density of CD68-positive cells ( D ) Kaplan–Meier OS curves according to the density of CD163-positive cells ( E ) Kaplan–Meier RFS curves according to PD-L1 expression in tumor cells ( F ) Kaplan–Meier RFS curves according to PD-L1 expression in stroma ( G ) Kaplan–Meier RFS curves according to the density of CD68-positive cells ( H ) Kaplan–Meier RFS curves according to the density of CD163-positive cells. (The density of CD68/CD163 above median = high CD68/CD163; The density of CD68/CD163 below median = low CD68/CD163; p -values obtained from Log-rank tests.).

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: Cancer Management and Research

Article Title: Tumor-Associated CD163 + M2 Macrophage Infiltration is Highly Associated with PD-L1 Expression in Cervical Cancer

doi: 10.2147/CMAR.S257692

Figure Lengend Snippet: Univariate Analysis of Overall and Recurrence-Free Survival

Article Snippet: After placing in a microwave oven for pretreatment (PD-L1: EDTA antigen repair solution for 30 min, pH 9; CD68 and CD163: sodium citrate buffer for 15 min, pH 6), endogenous peroxidase was inhibited by 3% H 2 O 2 for 30 min, then the sections were incubated with 10% normal goat serum for 40 min. Primary antibodies composed of rabbit anti-PD-L1 antibody 28–8 (diluted 1:100, ab205921, Abcam, Cambridge, UK), rabbi anti-CD68 antibody (diluted 1:50, BA3638, Boster, California, UK), or rabbit anti-CD163 antibody (diluted 1:200, bs-2527R, bioss, Beijing, China), were applied overnight in a moist chamber at 4°C.

Techniques: Expressing

Journal: EBioMedicine

Article Title: LDL Receptor Gene-ablated Hamsters: A Rodent Model of Familial Hypercholesterolemia With Dominant Inheritance and Diet-induced Coronary Atherosclerosis

doi: 10.1016/j.ebiom.2017.12.013

Figure Lengend Snippet: Analysis of plasma lipids and aortic and coronary atherosclerotic lesions in the WT and Ldlr +/− hamsters on the HCHF diet for 12 Weeks. (a and b) Plasma TC and TG were measured at the indicated time points in WT (n = 16) and Ldlr +/− (n = 20) hamsters on the HCHF diet for 12 weeks. The lesion size in the whole aorta was quantified in the mixed-sex hamsters. (c) Representative en face image of the whole aorta in the WT and Ldlr +/− hamster. (d–g) The lesion sizes in the whole aorta (D), aortic arch (e), thoracic aorta (f) and abdominal aorta (g) were quantified in the mixed-sex hamsters. (h) Representative Oil Red O staining of the aortic root in the WT and Ldlr +/− hamsters. (i) Lesion areas in the aortic root were quantified (WT, n = 16; Ldlr +/−, n = 20). ** denotes p < 0.01. (j) Immunohistochemical analysis of the atherosclerotic lesions in the WT and Ldlr +/− hamsters after 12 weeks on the HCHF diet. Representative staining of HE, CD68, VCAM1 and SMA in the lesion from the aortic root. (k) Representative image of the coronary atherosclerotic lesion in the WT and Ldlr +/− hamsters. (l) Semi-quantitative measurements of lesions in the coronary arteries of the WT (n = 16) and Ldlr +/− hamsters (n = 20). The arrows indicate positive staining. The black arrows indicate Oil Red O positive staining. Scale bar = 200 μm. All values are expressed the mean ± SEM.

Article Snippet: To characterize the atherosclerotic lesions, Immunohistochemistry of CD68, VCAM-1 and SMA was performed using cryo-sections of the hearts with a primary CD68 antibody (1:100 rabbit polyclonal IgG; BA3638, BOSTER), VCAM-1 antibody (1:100 rabbit polyclonal IgG; BA3840, BOSTER) and α-SMA antibody (1:100 rabbit polyclonal IgG; A03744, BOSTER).

Techniques: Clinical Proteomics, Staining, Immunohistochemical staining